외부 무기인 농도 변화에 의해 조절되는 pst pit 돌연변이 살모넬라주의 arg 및 art 유전자 전사
Transcriptional expression of arg and art genes in pst pit mutants of Salmonella Typhimurium according to extracellular inorganic phosphate levels
박윤미, 방일수,
소속 상세정보
박윤미 ( Park Yoon-Mee ) - 조선대학교 치의학전문대학원 미생물학 및 면역학교실
방일수 ( Bang Iel-Soo ) - 조선대학교 치의학전문대학원 미생물학 및 면역학교실
KMID : 0353320130370020082
Abstract
Purpose: Salmonella enterica serovar Typhimurium (S. Typhimurium) can cause salmonellosis, including gastroenteritis and systemic infection in animal hosts such as humans by diet contamination. To replicate and survive in an animal host, S. Typhimurium must compete against host cells for nutrients. Inorganic phosphate (Pi) is an essential element for all organisms and is used for cellular signal transduction and synthesis of various biomolecules. Thus, systems for uptake and homeostasis of Pi are required for cell metabolism. Uptake of Pi in S. Typhimurium is mainly regulated by components of the high-affinity adenosine triphosphate-binding cassette-transport system Pst and low-affinity transporter Pit. To identify genetic factors contributing to homeostasis of Pi, this study investigated the transcriptional profiles of Salmonella mutants lacking Pst and Pit transporters experiencing changes in extracellular Pi.
Materials and Methods: Transcriptome of pst pit mutant S. Typhimurium in response to low (0.1 mM) and high (10 mM) extracellular Pi were compared by cDNA microarray analysis, respectively.
Results: The results show that transcription of argI encoding ornithine carbamoyltransferase and artJ encoding arginine third transport system was significantly induced.
Conclusion: Data suggest a relationship between arginine metabolism and Pi homeostasis in S. Typhimurium.
키워드
Phosphate transporter proteins; Phosphate-specific transport protein system; Arginine; Salmonella
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